domingo, 2 de agosto de 2015

Genomic characterization of a persistent rubella virus from a case of Fuch' uveitis syndrome in a 73 year old man. - PubMed - NCBI

Genomic characterization of a persistent rubella virus from a case of Fuch' uveitis syndrome in a 73 year old man. - PubMed - NCBI



 2015 Aug;69:104-9. doi: 10.1016/j.jcv.2015.06.084. Epub 2015 Jun 19.

Genomic characterization of a persistent rubella virus from a case of Fuch' uveitis syndrome in a 73 year old man.

Abstract

BACKGROUND:

Many cases of Fuchs' uveitis have been associated with persistent rubella virus infection. A 73-year-old male patient with typical Fuchs' Uveitis Syndrome (FUS) first experienced heterochromia of the left eye at the age fourteen, when rubella was endemic in the US.

OBJECTIVES:

The purposes of this report are to describe the patient's FUS clinical presentations and to characterize the virus detected in the vitreous fluid.

STUDY DESIGN:

The patient underwent a therapeutic pars plana vitrectomy in May 2013. A real-time RT-PCR assay for rubella virus was performed on the vitreous fluid by Focus Diagnostics. Additional real-time RT-PCR assays for rubella virus detection and RT-PCR assays for generation of templates for sequencing were performed at the Centers for Disease Control and Prevention (CDC).

RESULTS:

The results from Focus Diagnostics were positive for rubella virus RNA. Real-time RT-PCR assays at CDC were also positive for rubella virus. A rubella virus sequence of 739 nucleotides was determined and phylogenetic analysis showed that the virus was the sole member of a new phylogenetic group when compared to reference virus sequences.

CONCLUSIONS:

While FUS remains a clinical diagnosis, findings in this case support the association between rubella virus and the disease. Phylogenetic analysis provided evidence that this rubella virus was likely a previously undetected genotype which is no longer circulating. Since the patient had rubella prior to 1955, this sequence is from the earliest rubella virus yet characterized.
Copyright © 2015 Elsevier B.V. All rights reserved.

KEYWORDS:

Fuchs’ uveitis syndrome; Genomic characterization of rubella virus; Rubella virus

PMID:
 
26209390
 
[PubMed - in process]

No hay comentarios:

Publicar un comentario