viernes, 22 de mayo de 2015

Actin Depletion Initiates Events Leading to Granule Secretion at the Immunological Synapse: Immunity

Actin Depletion Initiates Events Leading to Granule Secretion at the Immunological Synapse: Immunity

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Actin Depletion Initiates Events Leading to Granule Secretion at the Immunological Synapse

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5Present address: Research Center for Applied Science, Academia Sinica, Taipei 11529, Taiwan
6Present address: Department of Biological Sciences, University of Denver, Denver, CO 80208-9010, USA
7Present address: Department of Chemistry, Stony Brook University, Stony Brook, NY 11794-3400, USA
8Co-first author
9Co-senior author
Open Access
Open access funded by Wellcome Trust

Highlights

  • 4D imaging elucidates the order of events leading to secretion
  • Actin depletion initiates events leading to centrosome polarization and secretion
  • Lattice light-sheet imaging reveals a rearward flow of actin away from the synapse
  • Both centrosome and granules are delivered to an area of membrane depleted of actin

Summary

Cytotoxic T lymphocytes (CTLs) use polarized secretion to rapidly destroy virally infected and tumor cells. To understand the temporal relationships between key events leading to secretion, we used high-resolution 4D imaging. CTLs approached targets with actin-rich projections at the leading edge, creating an initially actin-enriched contact with rearward-flowing actin. Within 1 min, cortical actin reduced across the synapse, T cell receptors (TCRs) clustered centrally to form the central supramolecular activation cluster (cSMAC), and centrosome polarization began. Granules clustered around the moving centrosome within 2.5 min and reached the synapse after 6 min. TCR-bearing intracellular vesicles were delivered to the cSMAC as the centrosome docked. We found that the centrosome and granules were delivered to an area of membrane with reduced cortical actin density and phospholipid PIP2. These data resolve the temporal order of events during synapse maturation in 4D and reveal a critical role for actin depletion in regulating secretion.

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